What is the restriction site for the enzyme HaeIII?
Restriction Enzyme Cut Site: GG/CC.
What is the recognition site for HindIII?
Thermo Scientific HindIII restriction enzyme recognizes A^AGCTT sites and cuts best at 37°C in R buffer. See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes.
Which is the recognition site for restriction enzyme on DNA?
The restriction enzyme is a protein produced by bacteria that cleaves the DNA at specific sites. This site is known as the restriction site. The restriction enzymes protect the live bacteria from bacteriophages. They recognize and cleave at the restriction sites of the bacteriophage and destroy its DNA.
What do restriction enzymes recognize?
Restriction enzymes, also called restriction endonucleases, recognize a specific sequence of nucleotides in double stranded DNA and cut the DNA at a specific location. They are indispensable to the isolation of genes and the construction of cloned DNA molecules.
What is EcoRI restriction enzyme?
EcoRI is a restriction enzyme or restriction endonuclease. It cuts the DNA double helix at a specific site. This restriction enzyme was first isolated from E. coli. These restriction enzymes derive their names from the organisms where it was first isolated from.
What is the recognition sequence of Hind 3?
A/AGCTT
Hind III recognizes the sequence A/AGCTT and gene- rates fragments with 5´-cohesive termini (1). Compatible ends The enzyme is not known to have compatible ends. Isoschizomers The enzyme is not known to have isoschizomers.
What are the recognition sequence for EcoRI?
EcoRI recognizes the sequence GAATTC, and cuts both DNA strands between the G and the A nucleotides. Protruding from the cut ends will be single-stranded DNA “tails” having the sequences AATT.
How many restriction sites are there for EcoRI?
Note, after a reaction with the EcoRI enzyme, that the DNA of species A is cleaved into three fragments, corresponding to two EcoRI restriction sites, whereas that of species B is cleaved into four fragments, corresponding to three EcoRI restriction sites.
How do I find restriction sites?
First, work out the frequency of occurrence of the restriction site as 1-in-x bases, as explained in the example for the Intermediate level calculation. Then take the size of the DNA in kb (kilobases) and multiply by 1000 to get the size in bases. Divide this by x and round to the nearest whole number.
What is the recognition site for EcoRI?
The recognition site for EcoRI is a palindrome. It is a six nucleotide sequence containing GAATTC in 5′ to 3′ direction and the complementary strand contains CTTAAG in 3′ to 5′ direction. It cuts DNA after G↓AATTC, producing AATT sticky ends.
What is EcoRI restriction site?
Restriction site of EcoRI is a palindrome and it cuts DNA after G forming sticky ends with AATT. The complementary sequence of DNA for G/AATTC is CTTAA/G, where ‘/’ denotes the site where peptide bond is broken.
What is the recognition site of EcoRI?