What genes are regulated by NFKB?
NF-κB induces the expression of various pro-inflammatory genes, including those encoding cytokines and chemokines, and also participates in inflammasome regulation. In addition, NF-κB plays a critical role in regulating the survival, activation and differentiation of innate immune cells and inflammatory T cells.
How many subunits does NF KB have?
NF-κB is of course a family of related transcription factors; RelA/p65, c-Rel, RelB, p50 and p52 that may hetero- and homo-dimerise to form at least 12 different identified dimers. All NF-κB subunits contain a conserved Rel Homology Domain (RHD) that facilitates dimerisation and DNA binding.
What is NFKB signaling?
Nuclear factor kappa B (NF-κB) is an ancient protein transcription factor (Salminen et al., 2008) and considered a regulator of innate immunity (Baltimore, 2009). The NF-κB signaling pathway links pathogenic signals and cellular danger signals thus organizing cellular resistance to invading pathogens.
What does activation of NFKB do?
NF-κB target genes involved in inflammation development and progression. NF-κB is an inducible transcription factor. After its activation, it can activate transcription of various genes and thereby regulate inflammation.
How is NFKB activation measured?
Another common method to measure NF-κB activity at the transcriptional activation level is to use a gene reporter assay, which introduces an exogenous NF-κB consensus promoter sequence linked to a measurable ‘reporter gene’ such as luciferase (18).
What is NF KB Signalling?
What is the function of Nfkb?
NF-κB regulates the expression of a large array of genes involved in different aspects of atherosclerotic pathogenesis. In vascular endothelial cells, NF-κB mediates induction of pro-inflammatory cytokines, chemotactic factors and adhesion molecules, thereby promoting monocyte recruitment and disease progression.
How is NFKB pathway activated?
The primary mechanism for canonical NF-κB activation is the inducible degradation of IκBα triggered through its site-specific phosphorylation by a multi-subunit IκB kinase (IKK) complex. IKK is composed of two catalytic subunits, IKKα and IKKβ, and a regulatory subunit named NF-κB essential modulator (NEMO) or IKKγ.
What can activate Nfkb?
Activation process (canonical/classical) Activation of the NF-κB is initiated by the signal-induced degradation of IκB proteins. This occurs primarily via activation of a kinase called the IκB kinase (IKK).