How much does a HiSeq cost?
Table 1 Technical specifications of Next Generation Sequencing platforms utilised in this study
| Platform | Illumina MiSeq | Illumina HiSeq 2000 |
|---|---|---|
| Instrument Cost* | $128 K | $654 K |
| Sequence yield per run | 1.5-2Gb | 600Gb |
| Sequencing cost per Gb* | $502 | $41 |
| Run Time | 27 hours*** | 11 days |
What is HiSeq sequencing?
The HiSeq 2500 System is a powerful sequencing system with the flexibility to perform multiple applications. High-quality data using proven Illumina SBS chemistry has made it the instrument of choice for major genome centers and research institutions throughout the world.
What is HiSeq used for?
With a unique mix of high output, long reads, and paired-end sequencing, HiSeq is a powerful tool for de novo sequencing of species without a reference genome.
How many samples does Lane Illumina HiSeq have?
The answer to the specific question is that a HiSeq generates about 180-190 million reads per channel, so if your target is 50 million reads you can pool about 3-4 libraries if you are using that platform (HiSeq 2000 or 2500).
What is the difference between NovaSeq and HiSeq?
The Illumina NovaSeq provides a massive upgrade in sequencing throughput compared to the HiSeq 4000. There are more stringent library requirements and requires a larger sample size.
Is HiSeq discontinued?
The HiSeq 3000/HiSeq 4000 Systems have been discontinued. We will continue to provide full support of the instruments and supply the reagents through February 28th, 2023 (for the HiSeq 3000 System) or March 31, 2024 (for the HiSeq 4000 System).
How much does a HiSeq 4000 cost?
The HiSeq 4000, which will sell for $900,000 per instrument, can sequence up to 12 genomes, 100 whole transcriptome samples, or 180 exomes in three and a half days or less.
What is the difference between HiSeq and NovaSeq?
How many lanes does the HiSeq 4000 have?
Performance Parameters
| HiSeq 3000 System | HiSeq 4000 System | |
|---|---|---|
| Data Yield – 2 × 150 bp | 650–750 Gb | 1300–1500 Gb |
| Data Yield – 2 × 75 bp | 325–375 Gb | 650–750 Gb |
| Data Yield – 1 × 50 bp | 105–125 Gb | 210–250 Gb |
| Clusters Passing Filter (8 lanes per flow cell) | up to 2.5B single reads or 5B paired end reads | up to 5B single reads or 10B PE reads |
What do Illumina adapters do?
Adapters contain: Sequences that allow the library to bind and generate clusters on the flow cell (p5 and p7 sequences) Sequencing primer binding sites to initiate sequencing (Rd1 SP and Rd2 SP)