How does Bruker MALDI work?
MALDI-TOF MS determines the unique proteomic fingerprint of an organism, and matches characteristic patterns with an extensive reference library to determine the organism´s identity. Continuous expansion of the reference library ensures that a broad range of microorganisms can be identified easily.
How many species can the Bruker MALDI-TOF identify?
The MALDI Biotyper® CA System covers 488 clinically relevant bacteria and yeast species, representing more than 98% of the typical microbial identification workflow of clinical microbiology laboratories.
Why is TOF used with MALDI?
The type of a mass spectrometer most widely used with MALDI is the time-of-flight mass spectrometer (TOF), mainly due to its large mass range. The TOF measurement procedure is also ideally suited to the MALDI ionization process since the pulsed laser takes individual ‘shots’ rather than working in continuous operation.
What kind of instrument is TOF?
Time of flight (TOF) mass spectrometers are analytical instruments broadly used in life science research in areas such as drug discovery, microbiology, cell biology, and protein biochemistry. The TOF mass analyzer accelerates ionized sample particles as a beam or by relfection toward a detector.
How do you clean a Bruker MALDI plate?
Wet a tissue with water and wipe the upper surface of the MALDI target plate. 3. Place the MALDI target into a clean high-sided container and pour in enough 2-propanol to submerge the MALDI target plate. Place the container in the ultrasonic bath and sonicate for 10 minutes.
How does MALDI-TOF work microbiology?
MALDI-TOF measures the mass of molecules from a sample that has been embedded in a matrix by using a laser to ablate and desorb the molecules with minimal fragmentation.
What is TOF analysis?
Time-of-Flight Secondary Ion Mass Spectrometry (ToF-SIMS) is a surface-sensitive analytical method that uses a pulsed ion beam (Cs or microfocused Ga) to remove molecules from the very outermost surface of the sample. The particles are removed from atomic monolayers on the surface (secondary ions).
What are limitations of MALDI-TOF?
The greatest limitation of MALDI-TOF include low analytical sensitivity without prior culture and the discrimination of phyletically related microorganisms such as Shigella and Escherichia coli.
What types of sample are suitable for MALDI-TOF mass analysis?
Samples for MALDI analysis may be supplied either in solution or lyophilised. Ideally samples should be resuspended in an aqueous solution of 50% (v/v) acetonitrile, 0.1% (v/v) trifluoroacetic acid using ultra high purity water.
What are the advantages of MALDI?
Table 1
| Detection method | Advantages | Disadvantages |
|---|---|---|
| MALDI-TOF MS | • Fast • Accurate • Less expensive than molecular and immunological-based detection methods • Trained laboratory personnel not required | • High initial cost of the MALDI-TOF equipment |