How do you dilute antifoam 204?
Antifoam A Concentrate should be diluted with 3-10 parts of propylene glycol (aqueous) or vegetable oil (nonaqueous) with slow mixing. The product will be stable in the pH range of 5 to 9.
How do you dissolve antifoam?
Antifoam 204 is soluble in methanol, ethanol, toluene, xylene, perchloroethylene and cold water at temperatures below 15°C. It is insoluble in warm water and ethyleneglycol.
How do you use antifoam 204?
Antifoam 204 can be added to media prior to sterilization or sterilized individually by autoclaving and added to sterile fermenter media on an as-needed basis using an antifoam probe connected to a suitable pump.
What is the density of antifoam?
ANTIFOAM 204
| density | 1.01 g/cm3 at 25 °C(lit.) |
|---|---|
| storage temp. | room temp |
| form | emulsion (aqueous) |
How much antifoam do I add?
Usually add 3 to 5 times the dilution, up to 10 times. For the bulk type defoamer, it is generally diluted to an active content of 5 to 10%. If the foaming system has a good stirring and dispersing device, the emulsion type defoaming agent can be directly added without dilution.
How do you use an anti foaming agent?
Once the antifoam has entered the lamella, a lens is formed by the antifoam on the lamella and begins to spread. The progressive spreading process reduces the thickness of the lens, the shape of which is altered by movements in the foam. Stresses occur until the lens breaks and the foam lamella ruptures.
What is the pH of defoamer?
5-9
Silicone defoamers work best in the pH range of 5-9.
What causes foaming in bioreactor?
Foaming in bioprocesses Foam occurs in bioprocesses due to the introduction of gases into the culture medium, and is further stabilised by proteins produced by organisms in the culture[1]. Foam is made up of liquid lamellas which are full of gas.
Can you use too much defoamer?
If this foaming isn’t controlled, it can lead to not only a mess, but a loss of spray pressure, due to pump cavitation. Too much antifoam can cause the redeposit of the antifoam onto the panels by having it sucked-up through the pumps and sprayed through the nozzles.
How do you use antifoam?
Since the amount of the antifoaming agent added is small, in order to make the antifoaming agent uniformly dispersed, it should be added in a place where the foaming liquid is drastically shaken. Many occasions can be added by hand. If the blister is violent or for rapid defoaming, it can be sprayed with a spray gun.
What are the chemicals used in defoamer?
Typically, defoamers are inert chemicals. Defoamers are comprised of a liquid, such as mineral oil or silicone, and a hydrophobic solid, such as hydrophobic silica, ethylene-bis-stearamide, fatty acid, and/or fatty alcohol.
What is antifoam used for?
A defoamer or an anti-foaming agent is a chemical additive that reduces and hinders the formation of foam in industrial process liquids. The terms anti-foam agent and defoamer are often used interchangeably. Strictly speaking, defoamers eliminate existing foam and anti-foamers prevent the formation of further foam.
How do you reduce foam in a bioreactor?
Apart from the use of anti-foaming agents that can negatively affect fermentation bacteria, you may try to reduce foaming in the bioreactor by reducing the stirring speed and by increasing the pH to 7.5-8, since foaming generally occurs in the acidogenic fermentation.
Is antifoam toxic?
Antifoam and defoamer for fermentation is a non-toxic, non-silicone defoamer specially designed for fermentation process in distilleries.It is a combination of polyalkylene glycols and fatty acid esters.It is water dispersible and can be applied in natural state by aspersion or by automatic dosing pump.