How do you detect DNA damage response?
DNA breaks and lesions may be detected by PCR or using agarose gel electrophoresis (7). PCR is one of the most frequently used techniques for detecting DNA damage (7).
How does radiation therapy damage DNA?
Ionizing radiation directly affects DNA structure by inducing DNA breaks, particularly, DSBs. Secondary effects are the generation of reactive oxygen species (ROS) that oxidize proteins and lipids, and also induce several damages to DNA, like generation of abasic sites and single strand breaks (SSB).
What is homologous recombination pathway?
Homologous recombination (HR) comprises a series of interrelated pathways that function in the repair of DNA double-stranded breaks (DSBs) and interstrand crosslinks (ICLs).
What is DDR in oncology?
The DNA Damage Response (DDR) is one of the scientific platforms we are focusing on to improve the clinical paradigm in oncology. Our understanding of the role the DDR plays in cancer is enabling us to push our research further to target a broad range of cancers including difficult to treat or aggressive cancers.
Does radiation destroy lymph nodes?
Axillary lymph node dissection with radiation significantly increases the likelihood of developing lymphedema (20,27). The specific radiation technique impacts on lymphedema risk as well.
Which vitamin helps with DNA repair?
Vitamin C supplementation was potentially beneficial, because an increase in DNA repair incision capacity was observed, which was not seen in well-nourished subjects.
Can green tea repair DNA?
An antioxidant found in green tea may increase levels of p53, a natural anti-cancer protein, known as the “guardian of the genome” for its ability to repair DNA damage or destroy cancerous cells.
What are the three major mechanisms of DNA repair?
There are three types of repair mechanisms: direct reversal of the damage, excision repair, and postreplication repair. Direct reversal repair is specific to the damage.